![]() The ligand structure of the Capto adhere, HEA Hypercel, and PPA Hypercel resins is shown in Fig. Downstream processing of the Capto MMC pools showed that it is feasible to produce material with comparable purity to a process with affinity capture after two chromatography steps.Ĭapto MMC Mammalian cell culture fluid Monoclonal antibody Multimodal chromatography Selective capture.Ĭopyright © 2014 Elsevier B.V. Upon the discovery of the capability of the AEX/HIC mixed mode resins to remove IgG1 antibody fragment, specifically LHF, experiments were performed to determine the mechanism of removal. Buffer B: 100 mM acetic acid, 50 mM Na-phosphate, 20 mM Na-succinate, pH 8. Four separate elution strategies were identified to selectively recover the mAb and could be applied with minimal optimization to other mAb feedstocks. Capto MMC 12 3 12 3 Column: Tricorn 5/100 Resin: (A) Capto MMC (B) SP Sepharose Fast Flow Sample: human blood plasma diluted 5 times, 10 CV Buffer A: 100 mM acetic acid, 50 mM Na-phosphate, 20 mM Na-succinate, pH 5.0. This new MMC resin will be useful in future mAb manufacturing platforms comprising of a. Analysis of the DBC versus molecular properties showed that the mAb-ligand binding interaction was predominantly charge based. The ligand structure of the Capto adhere, HEA Hypercel, and PPA Hypercel resins is shown in Fig. In this platform, a chromatographic ligand (Capto MMC) was studied for the. Capto MMC is a multimodal cation exchanger with the properties of a weak cation exchanger. A range of dynamic binding capacities was observed for 10 feedstocks (24-53g/L) and two feedstocks had poor binding properties (<10g/L) despite load conditioning towards a more favorable pH. T13: Structure and Flow Properties of Monoliths. Effects of molecule size and resin structure on protein adsorption on multimodal anion exchange chromatography media. Twelve mAb HCCF feedstocks were examined for dynamic binding capacity (DBC) and then two representative feedstocks were selected to develop a systematic approach for elution buffer development. Capto MMC is a multimodal resin that contains a ligand with the potential to participate in ionic, hydrophobic, and hydrogen boding interactions with proteins and is coupled to a highly cross-linked agarose bead matrix. This proof-of-concept study examines the applicability of using multimodal chromatography to selectively capture recombinantly produced monoclonal antibodies (mAb) directly from harvested mammalian cell culture fluid (HCCF) with minimal optimization.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |